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KMID : 1005220080230030161
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Journal of Embryo Transfer
2008 Volume.23 No. 3 p.161 ~ p.166
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Factors Affecting the Motility and Fertility of Frozen-thawed Stallion Semen
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Park Yong-Soo
Park Hum-Dae
Jang Yong-Seok
Cho Gil-Jae
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Abstract
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The techniques for the collection, cooling and freezing of semen and artificial insemination of horses are not fully understood in Korea. We investigated the percentages of total motile (TM) and progressively motile (PM) sperms after the collection, cooling and freezing of stallion semen. The average volume of semen was 167 ml in Thoroughbred and 68 ml in Arab. The average numbers of spermatozoa in Thoroughbred and Arab were 104×10^6/㎖ and 86×10^6/㎖ respectively. The average percentages of TM and PM were 82.3% and 88.6% in Thoroughbred, and 61.4% and 82.6% in Arab, respectively. The average percentage of TM at 4 hr after cooling at 5℃ was significantly lower than that at 0 hr ($30.0±4.1% vs. 78.0±2.5%, p<0.05), but the percentage of PM was similar between 66.5 and 73.2% at 0, 1, and 4hr. The average percentage of frozen-thawed Thoroughbred semen frozen in MFR5 extender was 56.2%, which was significantly higher than that of the semen frozen in LE extender (average 32.9%, p<0.05). The percentage of TM in Arab was similar for semen frozen in MFR5 extender and LE extender (18.2% and 21.2%, respectively), but the percentage of PM was significantly higher in sperm frozen in MFR5 extender than in sperm frozen in LE extender (69.0% vs. 36.4%, p<0.05). Four mares were artificially inseminated by Thoroughbred frozen-thawed semen and one of them fertilized at 11 day after artificial insemination. In this study, the collection, cooling and freezing of equine semen were possible under domestic conditions.
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KEYWORD
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equine, semen, cooling, freezing, artificial insemination
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